藤茶粉和二氢杨梅素对2种水产致病菌体外抑菌研究

doi:10.14012/j.cnki.fjsc.2022.02.007

渔业研究 ›› 2022, Vol. 44 ›› Issue (2): 162-168.DOI: 10.14012/j.cnki.fjsc.2022.02.007

藤茶粉和二氢杨梅素对2种水产致病菌体外抑菌研究

张先姝¹，刘进雄¹，朱志¹，唐玖玖¹，张俊波^2,3*，印双红^1,3*，张孟琴^2,4

1. 铜仁学院，农林工程与规划学院，贵州铜仁 554300；
2. 铜仁学院，贵州省梵净山地区生物多样性保护与利用重点实验室，贵州铜仁 554300； 3.铜仁学院大健康学院，贵州铜仁 554300； 4.铜仁学院材料与化学工程学院，贵州铜仁 554300

收稿日期:2021-10-11 修回日期:2021-11-19 出版日期:2022-04-25 发布日期:2022-04-25
通讯作者: 张俊波（1984-），教授，博士，主要从事动物传染病研究.E-maiL：zhangjunbo666@126.com 印双红（1986-），副教授，硕士，主要从事感染与免疫研究.E-maiL：ysh_hong2006@163.com
作者简介:张先姝（2000-），女，汉族，在读本科生，主要从事中草药对水产细菌的抑制研究
基金资助:
贵州省科技支撑项目（黔科合支撑[2019]2348）；贵州省重点实验室项目（黔科合平台人才[2020]2003号）；铜仁市科技局科技支撑计划项目（铜市科研[2020]128号）

Study on in vitro bacteriostasis of ampelopsis powder and dihydromyricetin on two aquatic pathogenic bacteria

Received:2021-10-11 Revised:2021-11-19 Online:2022-04-25 Published:2022-04-25

摘要/Abstract

摘要： 试验研究藤茶成分对鱼类常见致病菌的体外抑制效果。选取藤茶粉和二氢杨梅素对维氏气单胞菌和嗜水气单胞菌进行体外抑菌试验，采用琼脂平板打孔法、试管二倍稀释法和平板培养法，测定抑制圈直径、最小抑菌浓度（MIC）和最小杀菌浓度（MBC）。结果显示，藤茶粉对维氏气单胞菌和嗜水气单胞菌的MIC和MBC均为6.25 mg/mL；二氢杨梅素对维氏气单胞菌和嗜水气单胞菌的MIC和MBC均为1.25 mg/mL。结果表明，藤茶粉和二氢杨梅素对维氏气单胞菌和嗜水气单胞菌均有较好的抑制效果，为藤茶成分作为饲料添加剂治疗水产细菌病提供了参考。

关键词: 藤茶粉, 二氢杨梅素, 维氏气单胞菌, 嗜水气单胞菌, 抑菌

Abstract: The inhibitory effect of Ampelopsis grossedentata components on common pathogenic bacteria in fish was studied in vitro. Ampelopsis powder and dihydromyricetin were selected for in vitro antibacterial test on Aeromonas veronii and Aeromonas hydrophila. The diameter of inhibition zone, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined by agar plate perforation method, tube double dilution method and plate culture method. The results showed that the MIC and MBC of ampelopsis powder against A. veronii and A. hydrophila were 6.25 mg/mL. The MIC and MBC of dihydromyricetin against A. veronii and A. hydrophila were 1.25 mg/mL. The results showed that ampelopsis powder and dihydromyricetin had good inhibitory effects on A. veronii and A. hydrophila, which laid a foundation for the treatment of fish bacterial diseases with A. grossedentata as feed additive.

Key words: ampelopsis powder, dihydromyricetin, Aeromonas veronii, Aeromonas hydrophila, bacteriostasis

中图分类号:

S917.1

张先姝刘进雄朱志唐玖玖张俊波印双红张孟琴. 藤茶粉和二氢杨梅素对2种水产致病菌体外抑菌研究[J]. 渔业研究, 2022, 44(2): 162-168.

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藤茶粉和二氢杨梅素对2种水产致病菌体外抑菌研究

Study on in vitro bacteriostasis of ampelopsis powder and dihydromyricetin on two aquatic pathogenic bacteria

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