养殖水体中甲苯咪唑的动态消除规律研究

doi:10.14012/j.cnki.fjsc.2023.03.010

渔业研究 ›› 2023, Vol. 45 ›› Issue (3): 297-303.DOI: 10.14012/j.cnki.fjsc.2023.03.010

养殖水体中甲苯咪唑的动态消除规律研究

钱卓真(), 李雷斌, 刘智禹, 汤水粉, 王丽娟, 姜琳琳, 陈宇锋, 位绍红, 余颖, 刘海新, 陈思, 罗方方

福建省水产研究所,福建省海洋生物增养殖与高值化利用重点实验室, 海洋生物种业技术国家地方联合工程研究中心,福建厦门 361013

收稿日期:2022-10-11 出版日期:2023-06-25 发布日期:2023-06-16
作者简介:钱卓真(1981—),女,助理研究员,博士,从事海洋环境污染物、农兽药残留分析及水产品质量安全研究。E-mail:qianzhuozhen@126.com
基金资助:
福建省海洋与渔业结构调整专项(2021HYJG02);福建省省属公益类科研院所基本科研专项(2020R1013002)

Study on the dynamic elimination pattern of mebendazole in aquaculture water

QIAN Zhuozhen(), LI Leibin, LIU Zhiyu, TANG Shuifen, WANG Lijuan, JIANG Linlin, CHEN Yufeng, WEI Shaohong, YU Ying, LIU Haixin, CHEN Si, LUO Fangfang

Key Laboratory of Cultivation and High-value Utilization of Marine Organisms in Fujian Province, National and Local Joint Engineering Research Center of Marine Biological Seed Industry Technology, Fisheries Research Institute of Fujian,Xiamen 361013,China

Received:2022-10-11 Online:2023-06-25 Published:2023-06-16

摘要/Abstract

摘要：

本文建立了高效液相色谱-串联质谱定量检测养殖水体中甲苯咪唑方法,方法定量限为0.5 μg/L,3个添加水平的平均回收率为79.0%~93.9%,日内相对标准偏差(RSD)为7.57%~8.27%,日间RSD为 7.43%~8.11%;分别以实际施药浓度15、50 μg/L作为受试浓度,开展室外水体中甲苯咪唑的消除实验。结果表明,室外养殖水体在自然光照下,甲苯咪唑的光降解速率遵循一级动力学规律,648 h后甲苯咪唑的光降解率可达80%以上;在15~18℃水温下,水体中甲苯咪唑消除半衰期为231.05 h;实验结束(648 h)时,施药浓度为50 μg/L的高浓度组水体中仍有6.4 μg/L甲苯咪唑。本研究结果可为水产养殖动物中甲苯咪唑的药代动力学研究提供基础数据。

关键词: 养殖水体, 甲苯咪唑, 光解, 动态消除

Abstract:

A high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method was developed for the quantitative determination of mebendazole in aquaculture water.The limit of quantification was 0.5 μg/L.The average recoveries of the three spiked levels ranged from 79.0% to 93.9%.The intra-day and inter-day relative standard deviations (RSDs) were 7.57%-8.27% and 7.43%-8.11%,respectively.The actual application concentrations of 15 μg/L and 50 μg/L were used as the tested concentrations for the elimination experiments of mebendazole in aquaculture water,respectively.The results showed that mebendazole was a photosensitive drug whose photodegradation followed the first-order kinetics.The photolytic degradation rate of mebendazole in outdoor aquaculture water under natural light was more than 80% after 648 h.The elimination half-life of mebendazole was 231.05 h at 15-18℃.At the end of the experiment (648 h),6.4 μg/L of mebendazole was still detected in the high concentration group(50 μg/L).Therefore,the results of this study could provide basic data for the pharmacokinetic study of mebendazole in aquaculture animals.

Key words: aquaculture water, mebendazole, photolysis, dynamic elimination

中图分类号:

钱卓真, 李雷斌, 刘智禹, 汤水粉, 王丽娟, 姜琳琳, 陈宇锋, 位绍红, 余颖, 刘海新, 陈思, 罗方方. 养殖水体中甲苯咪唑的动态消除规律研究[J]. 渔业研究, 2023, 45(3): 297-303.

QIAN Zhuozhen, LI Leibin, LIU Zhiyu, TANG Shuifen, WANG Lijuan, JIANG Linlin, CHEN Yufeng, WEI Shaohong, YU Ying, LIU Haixin, CHEN Si, LUO Fangfang. Study on the dynamic elimination pattern of mebendazole in aquaculture water[J]. Journal of Fisheries Research, 2023, 45(3): 297-303.

图/表 6

参考文献 19

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加标浓度/(μg/L) Spiked concentration	天数/d Days	回收率/% Recoveries (n=6)	日内精密度 Intra-day precision		日间精密度 Inter-day precision
加标浓度/(μg/L) Spiked concentration	天数/d Days	回收率/% Recoveries (n=6)	平均回收率/% Average recovery (n=6)	日内相对标准偏差/% RSD(n=6)	平均回收率/% Average recovery (n=18)	日间相对标准偏差/% RSD(n=18)
0.5	1	73.6、77.1、85.6、90.8、82.4、87.0	82.8	7.77	81.9	7.97
	5	82.4、88.6、83.1、70.6、73.9、75.1	79.0	8.63
	9	89.7、82.5、85.9、91.9、77.4、76.3	84.0	7.60
5.0	1	70.8、83.5、88.6、79.6、87.4、71.4	80.2	9.65	83.3	8.11
	5	86.1、86.2、79.1、77.9、90.2、92.0	85.3	6.70
	9	90.4、81.8、87.6、80.4、74.9、92.1	84.5	7.93
20.0	1	90.5、107、91.3、88.4、84.8、93.6	92.6	8.27	93.3	7.43
	5	84.5、88.6、90.1、96.1、101、103	93.9	7.81
	9	90.1、93.5、88.8、105、85.4、97.8	93.4	7.57

加标浓度/(μg/L) Spiked concentration	天数/d Days	回收率/% Recoveries (n=6)	日内精密度 Intra-day precision		日间精密度 Inter-day precision
加标浓度/(μg/L) Spiked concentration	天数/d Days	回收率/% Recoveries (n=6)	平均回收率/% Average recovery (n=6)	日内相对标准偏差/% RSD(n=6)	平均回收率/% Average recovery (n=18)	日间相对标准偏差/% RSD(n=18)
0.5	1	73.6、77.1、85.6、90.8、82.4、87.0	82.8	7.77	81.9	7.97
	5	82.4、88.6、83.1、70.6、73.9、75.1	79.0	8.63
	9	89.7、82.5、85.9、91.9、77.4、76.3	84.0	7.60
5.0	1	70.8、83.5、88.6、79.6、87.4、71.4	80.2	9.65	83.3	8.11
	5	86.1、86.2、79.1、77.9、90.2、92.0	85.3	6.70
	9	90.4、81.8、87.6、80.4、74.9、92.1	84.5	7.93
20.0	1	90.5、107、91.3、88.4、84.8、93.6	92.6	8.27	93.3	7.43
	5	84.5、88.6、90.1、96.1、101、103	93.9	7.81
	9	90.1、93.5、88.8、105、85.4、97.8	93.4	7.57

采集时间/h Collection time	浓度/(μg/L) Concentration
采集时间/h Collection time	0 h	72 h	120 h	168 h	216 h	288 h	384 h	504 h	648 h
低浓度组Low concentration group	12.6	10.4	8.3	5.4	3.7	3.1	2.6	2.0	1.8
高浓度组High concentration group	41.0	36.7	31.6	29.2	22.6	13.3	10.8	8.6	6.4

采集时间/h Collection time	浓度/(μg/L) Concentration
采集时间/h Collection time	0 h	72 h	120 h	168 h	216 h	288 h	384 h	504 h	648 h
低浓度组Low concentration group	12.6	10.4	8.3	5.4	3.7	3.1	2.6	2.0	1.8
高浓度组High concentration group	41.0	36.7	31.6	29.2	22.6	13.3	10.8	8.6	6.4

养殖水体中甲苯咪唑的动态消除规律研究

Study on the dynamic elimination pattern of mebendazole in aquaculture water

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