关键词: 尼罗罗非鱼, ISSR, 遗传分析, 分子标记, Nile tilapia, ISSR, genetic analysis, molecular marker

Abstract: ISSR analysis was conducted to investigate the genetic relationships of five Nile tilapia（population GF, NLⅠ, NLⅡ, NLⅢ and NLⅣ）. Out of 81 ISSR primers , 15 primers generated highly reproducible and polymorphic DNA fragments. The genetic trees were constructed based on ISSR amplification fingerprintings using unweighted pairgroup method for arithmetic averages analysis (UPGMA) methods. NLⅢ and NLⅣ strains formed one branch first, then grouped with GF population. NLⅠand NLⅡ formed a separate branch. This shows that: ①GF population separated from other Nile tilapia populations. ②The 500 bp band amplified by primer UBC835 could distinguish from GF and other Nile tilapia.