琼脂及琼脂糖中3,6-内醚半乳糖的测定

福建水产 ›› 2012, Vol. 34 ›› Issue (5): 370-374.

琼脂及琼脂糖中3,6-内醚半乳糖的测定

李龙¹,苏永昌²,吴成业²

1. 福建农林大学
2. 福建省水产研究所

收稿日期:2012-06-07 修回日期:2012-06-28 出版日期:2012-10-25 发布日期:2012-10-22
通讯作者: 吴成业

Determination of 3,6-anhydro galactose in agar and agarose

Received:2012-06-07 Revised:2012-06-28 Online:2012-10-25 Published:2012-10-22
Contact: wu chengye

摘要/Abstract

摘要： 本文研究了间苯二酚法对琼脂及琼脂糖样品中的3,6-内醚半乳糖的测定方法。实验中，根据间苯二酚试剂与3,6-内醚半乳糖和果糖的浓度在0.25 umol/mL以下时，具有近乎相同的标准比色曲线的原理，分别对测定波长、显色温度、显色时间和比色时间等检测条件进行优化。结果显示，用间苯二酚法测定3,6-内醚半乳糖的最佳检测条件为：测定波长554 nm、显色温度80℃、显色时间15 min；在该条件下，对琼脂及琼脂糖样品中的3,6-内醚半乳糖进行测定，方法的相对偏差为0.94%～1.27%，加标回收率为105.4%～111%。表明该方法操作简便，具有较高的准确性和重复性。

关键词: 3,6-内醚半乳糖, 琼脂, 琼脂糖, 3,6-anhydro galactose, agar, agarose

Abstract: This paper studied the determination of resorcinol method on agar and agarose samples of 3,6-anhydro galactose and optimize the determination wavelength, color temperature, color time and colorimetric time. the results show that the optimum condition is determination of wavelength of 554nm,color temperature 80℃,reaction time 15min; under this condition, agar and agarose samples of 3,6-anhydro galactose were measured ,the relative deviation of 0.94% to 3.0%, and the spiked recovery of 105.4% to 111%. It shows that the method is simple, high accuracy and repeatability.

李龙苏永昌吴成业. 琼脂及琼脂糖中3,6-内醚半乳糖的测定[J]. 福建水产, 2012, 34(5): 370-374.

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[2]	李龙苏永昌吴刚吴成业. 几种琼脂糖提取及改性方法概述[J]. 福建水产, 2013, 35(1): 73-77.