Preliminary study of artificial propagation technology of Andrias davidianus

Artificial propagation technology of Andrias davidianus was studied under experimental conditions in early July. The experiments were conducted in the cave with 200m length, 5.2m width and 4.5m height, and artificial cave for Andrias davidianus was made of flagstones and pebbles in breeding ponds with their area 6 m2and 0.48 m2 in this cave. The parent Andrias davidianus was 5-7 years old second filial generation. The culture condition for parent Andrias davidianus was as follows: temperature, 19.0℃～20.5℃, pH, 7.2～7.3，DO, 5.8～7.4mg/L, light intensity<300Lux. LRH-A2 and HCG were injected into the abdominal cavity for the artificial reproduction of parent Andrias davidianus, and their dosages (for male and female individuals) 10ug/㎏～20ug/㎏ and 1000IU/㎏～2000IU/㎏, respectively. During the experiment, a total 26 female and 24 male Andrias davidianus were involved. After 4 days of hormone injection, 962 fertilized eggs were collected from 3800 eggs of 4 female individuals, and 172 larvae were hatched by 35-38d hatching, and the hatching rate was 18%.