5-羟色胺和氨海水对钝缀锦蛤解剖卵母细胞的体外促熟作用

Effect of 5-HT and ammonia seawater on maturation of oocyte stripped from Tapes conspersus

摘要: 采用解剖卵母细胞体外浸泡促熟的方法,研究了5-羟色胺(5-HT)和氨海水对钝缀锦蛤(Tapes conspersus)卵母细胞的促熟作用。结果表明,采用一定浓度的5-HT和氨海水浸泡均能够显著诱导钝缀锦蛤卵母细胞的生发泡破裂(P＜0.05),5-HT和氨海水浸泡的卵母细胞受精率相差不大(P＞0.05)。其中,10 μmol/L 5-HT浸泡卵母细胞60 min获得的受精率最高,达到48.57%;0.020%氨海水浸泡卵母细胞45 min获得的受精率最高,达到50.75%,但畸形较多。

Abstract: To construct the artificial breeding method of Tapes conspersus,effects of 5-hydroxytryptamine(5-HT)and ammonia seawater on the maturation of oocytes were studied by soaking the dissected oocytes in vitro.Results showed that both immersion with ammonia seawater and 5-hydroxytryptamine significantly increase the germinal vesicles breakdown in Tapes conspersus (P＜0.05).However,the fertilization rate showed no significantly different between oocytes soaked in 5-hydroxytryptamine and ammonia seawater.The fertilization rate of oocytes treated with 10 μmol/L 5-HT for 60 min was 48.57%.The fertilization rate of oocytes treated with 0.020% ammonia seawater for 45 min was slightly higher(about 50.75%),whereas there were many malformations.