Abstract: 24 stations were set up in Minjiang River Estuary in November 2018(dry season)and September 2019(rainy season).The contents and temporal-spatial distribution characteristics of 88 antibiotics in surface water of Minjiang River Estuary were analyzed by solid phase extraction and liquid chromatography tandem mass spectrometry(HPLC-MS/MS),and the ecological risk of antibiotic pollutants was also evaluated.Results showed that,1)17 antibiotics of 5 categories(sulfonamides,macrolides,quinolones,chloramphenicols,nitroimidazoles and others)were detected at 24 stations in Minjiang River Estuary,and the total amount varied from 26.31 to 155.39 ng/L.2)In term of spatial distribution,the total amount of antibiotics did not change much in the upper reaches of Minjiang River Estuary,North Port and South Port,but it showed an obvious downward trend in the lower reaches.Different kinds of antibiotics displayed relatively different temporal and spatial distribution characteristics.Both the detected types and content of antibiotics in dry season were significantly higher than those in rainy season.3)Based on the results of ecological risk assessment,sulfamethoxazole had high ecological risk,erythromycin and lincomycin had medium ecological risk in the dry season;erythromycin showed moderate ecological risk in rainy season.According to results of mixed risk entropy,most stations in Minjiang River Estuary area were around the ecological high-risk threshold,which needed further attention and strengthening management.