Abstract: In order to provide scientific basis for the nutritional evaluation of Opsariichthys bidens muscle in Min River,the contents of routine nutrition components,fatty acids and amino acids in muscle were determined and analyzed by biochemical method.The results showed that the contents of water,crude protein,crude fat and crude ash in O.bidens muscle from Min River were(75.20±0.14)%,(22.20±0.85)%,(3.15±0.02)% and(1.32±0.01)%,respectively.17 kinds of amino acids were detected in muscle,7 of which were human essential amino acid,accounting for 35.92% of total amino acids(TAA)and 35.46% of umami amino acids(UAA)in TAA.The amino acid score(AAS)of EAA was more than 1.0 except Met+Cys,the chemical score(CS)was more than 0.8 except Met+Cys,the first limiting amino acid was Met+Cys,and the essential amino acid index(EAAI)was 81.37.The content of trace elements in muscle from high to low was K＞P＞Ca＞Na＞Mg＞Zn,of which the calcium-phosphorus ratio was 1.00∶4.49.To sum up,the O.bidens from Min River was a kind of high quality economic freshwater fish with good edible value and health care function.