Abstract: In order to explore the best method of heterologous migration of Leuciscus merzbacheri,and actively carry out the protection of germplasm resources,cage net,river net and purse seine were used to collect fish and put them into three kinds of temporary ponds:micro-soil pond,cement pond and net cage in the present study.The seeds were transported to the Aquatic Wildlife Rescue Center of Xinjiang for a long distance using canvas water tanks,iron water tanks and fish fry bags,and were treated with sodium chloride,povidone iodine and potassium permanganate for domestication.The results showed that the survival rate of purse seine fishing was the highest(97.47%),that of cage culture was the best(92.86%),that of transportation of fish fry bag with oxygen and cooling was the highest(97.17%).Using 15-20 mg/L potassium permanganate solution and medicated bath for 20-30 minutes,the effect of sterilization and wound healing could be improved,and the number of survivors could be ensured.A total of 534 original species were collected in three years,195 were domesticated and preserved,and the final survival rate was 36.52%.The successful relocation laid a good foundation for the future large-scale propagation of seedlings.