Abstract: Based on the indices of fluidity,uniformity,sedimentation coefficient and cost coefficient,the optimal formula of softgel inner fill was determined as Heliocidaris crassispina gonad oil:wheat-germ oil:α-tocopherol with mass ratio of 3:6:1.Based on the indexes of viscosity,hardness and disintegration time,the softgel wall formula was optimized as gelatin:deionized water:glycerol:polyethylene glycol 400:ethylparaben with mass ratio of 1:1.1:0.6:0.05:0.002 5 by using the single-factor method and orthogonal experimental design.The softgels were manufactured according to the above-mentioned optimal formulas by utilizing the softgels encapsulation machine with rotary pressing method.The moisture contents(0.20±0.016)%,set quantity deviations(≤ 8%),disintegration times(11.31±0.25) min and microbes amount of the softgels of H.crassispina gonad oil complex had met the criteria of 0103 Capsules,2015 year edition of the Pharmacopoeia of the People's Republic of China.Aiming to provide novel dietary lipid products from marine resources as well as related manufacturing technology parameters,this study has provided scientific data and theoretical projections for bio-diversified application of dietary lipids and better development of dietary lipids industry in China.