Abstract: Pyropia haitanensis is an economically important cultivated seaweed in China. Conchospore releasing is a critical event of artificial cultivation of Pyropia,consequently the amount of conchospore released is very important to the success of the seedling collection. Based on an orthogonal design with 3 factors and 5 levels,the effects of temperature,salinity and light intensity on the conchospore releasing of P.haitanensis were studied.The results showed that temperature had a significant effect on the amount of conchospore released (F=8.584,P =0.002<0.05);salinity and light intensity had no significant effects on the amount of conchospores (F=1.073,P=0.412>0.05;F=0.585,P=0.680>0.05).Analysis of variance showed that temperature had an extremely significant effect on the amount of conchospore released from P.haitanensis (F=9.215,P<0.01).Temperature was the first-order main effect,and the second-order interaction effect with salinity and light intensity was not significant (P>0.05).The optimal environmental conditions for the release of conchospore were as follows:temperature of 26℃,salinity of 1.018~1.023,and light intensity of 5 000~8 000 Lux.