Abstract: The experiment was conducted for 60 days in order to determine the effects of different feeding frequencies (1 time per day,2 times per day,and once every 2 days) on the growth and plasma anti-stress enzyme activities of the hybrid grouper (Epinephelus fuscoguttatus ♀×E.lanceolatus ♂) with initial weight of(297.79±59.94)g at water temperature of(29.96±0.40)℃,reared with artificial formulated in industrial recirculating aquaculture systems.The results showed that the survival rate of the fish fed once every 2 days (99.39±0.01)% was significantly higher than those of the fish fed once per day (96.75±0.66)% and twice a day (97.08±0.66)% (P<0.05).There were not significant effects on the specific growth rate (SGR=1.05~1.19%/d),condition factor (CF=1.63~1.77),relative weight gain (RWG=187.65%~205.04%),daily weight gain (DWG=4.33~5.00 g/d),coefficient of size variation (SV=14.50~18.30),food intake (FI=1.07~1.24%/d),food conversion efficiency (FCE=81.77%~87.38%) and food conversion ratio (FCR=1.15~1.25) among the three groups (P>0.05).The plasma anti-stress enzyme activities (i.e.AKP=8.07~8.26 King/100mL,CAT=1.92~2.52 U/mL and SOD=23.39~24.34 U/mL) of the fish was not significantly different in all treatments (P>0.05).Under the experimental conditions,the feeding frequency of once every two days could be used to improve the survival rate of the hybrid grouper (Epinephelus fuscoguttatus ♀×E.lanceolatus ♂) in industrial recirculating aquaculture systems.