Abstract: Clown fish(Amphiprion frenatus) was one of the most popular marine ornamental fish,and its market share was increasing in aquaculture industry.Astaxanthin could improve the growth rate and immune response of fish,and digestive enzyme could affect the growth performance of fish.This study analyzed the effect of dietary astaxanthin on the digestive enzyme activity of clown fish.90 juveniles of 30 days-post-hatch with the average weight and length of(4.88±0.32) g and(4.85±0.32) cm were divided into 5 groups,which was possessed of the same 3 replicates,and were fed with basal diet containing 0,2‰,4‰,6‰ and 8‰ astaxanthin for 20 days,respectively.The results indicated that dietary astaxanthin increased the digestive enzyme activities of clown fish.When dietary astaxanthin content was 4‰,the activities of anterior intestine protease,lipase,amylase and posterior intestine amylase were highest(P<0.05).When dietary astaxanthin content was 6‰,the activities of stomach lipase and amylase were highest(P<0.05).When dietary astaxanthin content was 8‰,the activities of posterior intestine protease,amylase and pepsase were highest(P<0.05).In conclusion,the digestive enzyme activities of A.frenatus could be effectively improved through feeding basal diet with 4‰~8‰ astaxanthin.