Abstract: In our paper the semi quantitative rapid detection kits of tetrodotoxin which developed at the Fisheries Research Institute of Fujian were applied in the artificial breeding of Fugu bimaculatus. We detected the tetrodotoxin of parent fishes, fertilized eggs, embryo and juveniles of Fugu bimaculatus in different breeding groups.The results showed the tetrodotoxin of tissue and organ with parent fishes was significant different from each other in different breeding groups.The tetrodotoxin of eye ball and testis in parent fishes were negative in detection, but on the other hand, the skin, muscle, liver and ovaries were positive with different detection rate which were 5.4%, 16.2%, 40.5% and 40.5%, respectively.Our experimental data had indicated that the skin and liver of parent fishes were positive in artificial breeding groups, and the positive detection rates of the liver and ovaries of parent fishes in wild groups were higher than artificial breeding groups.The fertilized eggs with female parent in wild groups were both positive in tetrodotoxin detection, and the embryo was positive in earlier state but negative in later state.The fertilized eggs and embryo with female parent in artificial breeding groups were both negative in tetrodotoxin detection.The juveniles with female parent in wild groups were positive in earlier state but negative in later state.The juveniles with female parent in artificial breeding groups were negative in tetrodotoxin detection.