Abstract: Supercritical fluid extraction as a new "green" extraction and separation technology has several advantages such as high separation efficiency and dissolving ability. It can maintain the biological activity of products, increase the extraction rate and purity of products etc. And the technique's operation is simple and feasible. The basic principles and characteristics of supercritical fluid extraction technology were introduced and its research progress of extracting bioactive substances from marine fisheries was reviewed, and its application prospects in the field were foreseen.