Abstract: Effect of different feeds on activity of digestive enzymes in Epinephelus coioides was examined in this article. 1500 Epinephelus coioides were randomly divided into three groups that two were replicated in each group, and were fed with three different feeds for 50 days, and then five Epinephelus coioides were randomly selected from each control to detect digestive enzyme activity. The results showed that different types and ingredients of feeds had significant effect on the digestive enzymes activity of Epinephelus coioides. In this experiment, No.2 feed had the highest amylase activity, No.3 feed had the highest overall protease activity, and fat was digested mainly in the intestine, three lipase activities in the intestine were not different obviously.