Abstract: In this study, enzymatic, field sampling and other methods were used to study pancreatic morphology and several biochemical component content changes in spermary and liver of Marsupenaeus japonicus during the spermary development. It intended to illustrate in the natural state, the growth and decline and transfer condition of nutrition substances in the tissues and organs of Marsupenaeus japonica broodstock in reproductive process. The results showed that in the natural state of the Taiwan Strait waters, Marsupenaeus japonicus, testis index from the phase I 0.010±0.001~IV period of 0.017±0.002 was gradually increased (P<0.05); liver and pancreas fell from Phase I of 0.021±0.002~to stage IV 0.025±0.003, while also showed increasing trend, but little change (P>0.05). Glucose, cholesterol and triglyceride levels (mg/dl) changed as follows:In the testis, is 14.85±5.40~4.75±3.17,7.69±2.47~16.46±6.79 and 15.85±4.56~36.78±8.12; in the liver pancreas, is 10.95±2.60~22.24±5.76,27.12±9.11~202.62±77.26 and 42.87±13.39~116.99±24.57; in the hemolymph, is 6.38±1.44~11.25±3.08,53.77±32.10~155.32±62.55 and 13.67±7.06~79.56±38.19. This paper also showed that during the development of Marsupenaeus japonicus spermary, how gonad、pancreas and liver changeed in morphological area. The situations of rise and fall with the transfer in liver, pancreas, blood, lymph, testis glucose, cholesterol and triglyceride were also discussed in detail.