Abstract: Electrical stimulation can induce muscular contraction.The spermatophores of Cherax destructor were ejected by electrical stimulation around the coxa of the fifth paraeiopod.In order to obtain more spermatophores,the conditions(voltage,frequency and duration) of electrical stimulation,without injury to crayfish,were optimized step by step.The results showed that the optimum conditions were voltage as 45 V,frequency as 32 Hz and duration as 4 s,which could get 75% spermatophore extrusion rate.The cellular morphology of spermatozoa in the spermatophore was normal.There were 5.2×10⁴ to 1.13×10⁶ spermatozoa in each spermatophore. This paper was important for providing technical support to artificial propagation of C.destructor.