Abstract: Hierarchical microbial purification,increasing oxygen aeration,circulating water breeding such as effective temperature control system of the Anguilla mormorata seedling cultivation tests have been carried out. Eel seedling density of livestock for 3 000 ind./m² and 3 050 ind./m²,about 5~6 times of the traditional model. Test results showed that within the scope of the water temperature 27~30℃,the individual weight 0.15~0.20 g (0.17 g) of the Anguilla mormorata seedling breeding,after 71 d development,individual average weight is 2.95 g,17.7 times weight,daily increasing weight 0.04 g,eel survival rate of seedling cultivation,98.81% higher than that of traditional cultivation pattern,water earthworms feed coefficient is 7.14. Tests show that factory circulating water aquaculture system is applicable to the Anguilla mormorata seedling cultivation,and has advantages of energy conservation and emissions reduction,It can provide a reference for the development of new varieties of eel aquaculture and environmental friendly eel seedling mode.