Abstract: The thesis generalized several methods of removing proteins and eliminating pigment from polysaccharide, and several kinds of principles and methods that classify and purify sulfated polysaccharides, including organic solvent precipitation, quaternary ammonium salt precipitation,ultrafiltration technology, ion exchange chromatography and gel chromatography, then summarized the analytical methods of sulfated polysaccharides in terms of sulfate radical, relative molecular weight, total sugar, purity and structure. At last, the thesis put forward to further research structure-function relationship of sulfated polysaccharides.