Abstract: Random amplified polymorphic DNA (RAPD) technique was applied to assess the relationship of genetic structure and species diversity between hybrids F1 and their parents. The RAPD fingerprints of hybrids F1 and their parents were obtained with RAPD analysis using 90 random primers, of which 35 generated polymorphic products. The results showed that the genetic distance between hybrids F1 and Channa argus(♀) or C.maculata(♂) were closer than between their parents, it meant hybrids F1 were the hybrid later generations of their parents. The polymorphic proportion of F1 hybrids 47.9% and heterozygosity of H=0.122 could be the genetic breeding standard to measure the genetic diversity of the species progeny.