Abstract: Focus on the detection problems of colistin, bacitracin and virginiamycin drug residues, this paper introduces different methods at home and abroad to detect these three kinds of substances in food of animal origin and feed,analyses and compares the advantages and disadvantages of microbiological,ELISA,capillary electrophoresis,HPLC and LC-MS.Finally HPLC and LC-MS are considered as the suited methods to quantitative and confirmatory analyse the residues of these substances in aquatic products.