5个尼罗罗非鱼群体遗传关系的ISSR分析

ISSR analysis on genetic relationships of five Nile Tilapia O.niloticus populations

摘要: 采用ISSR分子标记技术,对吉富尼罗罗非鱼群体(GF)、尼罗罗非鱼群体Ⅰ(NLⅠ)、尼罗罗非鱼群体Ⅱ(NLⅡ)、尼罗罗非鱼群体Ⅲ(NLⅢ)和尼罗罗非鱼群体Ⅳ(NLⅣ)的遗传多样性进行分析。用81个ISSR引物对5个尼罗罗非鱼群体进行扩增,15个ISSR引物获得多态片段。根据Nei's遗传距离矩阵构建了5个尼罗罗非鱼群体的遗传关系树状图。UPGMA聚类图表明:NLⅢ群体和NLⅣ群体最先聚在一起;其次二者再与GF群体聚在一起;NLⅠ群体与NLⅡ群体聚在一起。分析结果显示:GF群体与其它4个罗非鱼群体区分较明显;用UBC835扩增的500 bp片段为GF所特有,可作为区别GF和其它尼罗罗非鱼的分子标记。

Abstract: ISSR analysis was conducted to investigate the genetic relationships of five Nile tilapia（population GF, NLⅠ, NLⅡ, NLⅢ and NLⅣ）. Out of 81 ISSR primers , 15 primers generated highly reproducible and polymorphic DNA fragments. The genetic trees were constructed based on ISSR amplification fingerprintings using unweighted pairgroup method for arithmetic averages analysis (UPGMA) methods. NLⅢ and NLⅣ strains formed one branch first, then grouped with GF population. NLⅠand NLⅡ formed a separate branch. This shows that: ①GF population separated from other Nile tilapia populations. ②The 500 bp band amplified by primer UBC835 could distinguish from GF and other Nile tilapia.