Abstract: Combined with the active standards at home and abroad, especially with the active standards of Chinese ocean area, the research to find the analytic method of Fe, Mn, and Ni in the marine organisms was conducted. In this research, three sides were studied to get the normal standard method. The Cacl2 was joined in the samples to clear up the interferential of FLAA, and the admixture of Pd(NO3)2 and Mg(NO3)2 were joined in the samples to clear up the interferential of GFAA. The normal assisted acid digestion or the microwave as— sisted acid digestion of adding the admixture of HNO3 and H2 O2, and at the preparation temperature, then Fe, Mn were analyzed by FLAA or Mn, Ni were analyzed by GFAA. In order to validate the method by the test, the measured values of Fe, Mn, and Ni were in the ranges of reference value. Finally we can get the normal standard method in the Chinese ocean area.