琼脂及琼脂糖中3,6-内醚半乳糖的测定
Determination of 3,6-anhydro galactose in agar and agarose
-
摘要: 本文研究了间苯二酚法对琼脂及琼脂糖样品中的3,6-内醚半乳糖的测定方法。实验中,根据间苯二酚试剂与3,6-内醚半乳糖和果糖的浓度在0.25μmol/mL以下时,具有近乎相同的标准比色曲线的原理,分别对测定波长、显色温度、显色时间和比色时间等检测条件进行优化。结果显示,用间苯二酚法测定3,6-内醚半乳糖的最佳检测条件为:测定波长554 nm、显色温度80°C、显色时间15 min;在该条件下,对琼脂及琼脂糖样品中的3,6-内醚半乳糖进行测定,方法的相对偏差为0.94%～1.27%,加标回收率为105.4%～111.04%。表明该方法操作简便,具有较高的准确性和重复性。Abstract: This paper studied the determination of resorcinol method on agar and agarose samples of 3,6-anhydro galactose and optimize the determination wavelength, color temperature, color time and colorimetric time. the results show that the optimum condition is determination of wavelength of 554nm,color temperature 80℃,reaction time 15min; under this condition, agar and agarose samples of 3,6-anhydro galactose were measured ,the relative deviation of 0.94% to 3.0%, and the spiked recovery of 105.4% to 111%. It shows that the method is simple, high accuracy and repeatability.
下载:
