胶体金免疫层析法快速检测水产品中呋喃妥因代谢物残留

Gold immunochromatographic assay for rapid determination of nitrofurantoin metabolite residues in aquatic products

摘要: 采用胶体金免疫层析技术（GICA）测定水产品中的呋喃妥因代谢物残留，并用液相色谱-串联质谱（LC-MS/MS）对实验结果进行确证。结果显示，胶体金免疫层析法的检测限为1.0 μg/kg，假阳性率不大于5%，假阴性率为0；对实际样本的检测结果与LC-MS/MS法一致。研究表明，胶体金试纸条法操作简便、快速，成本低，为水产品中呋喃妥因代谢物的残留检测提供了快速筛选方法，而LC-MS/MS法灵敏度高，结果准确，适用于阳性样本的确证和精确定量。

Abstract: Gold immunochromatographic assay (GICA) was used to determine the nitrofurantoin metabolite (AHD) residues in aquatic products, and also the liquid chromatography-tandem mass spectrometry (LC-MS/MS) was used to validate the results. The detection limit of GICA was 1.0 μg/kg. The false positive rate was less 5%, and false negative rate was 0. The results of the two methods for aquatic products were consistent. The results showed that GICA had the advantages of convenient operation, rapidity and low cost, which provided a rapid method for the screen of AHD in aquatic products, while LC-MS/MS was accurate and suitable for determination of positive samples.