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    基于响应面方法的南极磷虾油提取工艺及其脱砷效果研究

    Antarctic krill oil extraction process and arsenic removal effect based on response surface method

      摘要
    • 摘要:
      目的 建立南极磷虾油提取与脱砷的最佳工艺，提高南极磷虾油产品的安全性，为高品质南极磷虾油的工业化生产奠定基础。
      方法 本文以冷冻南极磷虾粉为原料，采用响应面法优化南极磷虾油的提取工艺，利用原子荧光光度计测定法评价5种吸附材料（Cleanert Silica、Cleanert Alumina N、Cleanert S C18、Cleanert Alumina A、NH2氨丙基吸附柱）对南极磷虾油中砷的脱除效果。在单因素试验基础上，以南极磷虾油得率为响应值，选择乙醇浓度、液料比、超声时间为自变量，对提取的南极磷虾油进行工艺优化，并对其进行脱砷处理。
      结果 响应面法建立回归方程：X得率=19.02−0.30A＋0.68B＋0.92C−0.24AB−0.19AC＋0.49BC−1.11A2−1.04B2−1.29C2，模型P＜0.000 1，失拟项P>0.05，表明该模型极显著且比较稳定，通过模型分析得最佳工艺条件：乙醇浓度91.1563%、液料比8.689 2 mL/g、超声时间47.473 4 min。考虑实际应用的可行性，将条件修正为：乙醇浓度91%、液料比8 mL/g、超声时间47 min，得到实际南极磷虾油的得率为18.36%±0.58%，与模型理论预测值18.62%基本一致。以活性氧化铝为介质的Cleanert Alumina A吸附柱可脱除南极磷虾油中82.61%±4.63%的总砷。
      结论 基于响应面法得到的南极磷的Cleanert Alumina A吸附柱可作为未来南极磷虾油脱砷的理想材料。

       

      Abstract:
      Objective The present study aims to establish the best technology of extraction and arsenic removal of Antarctic krill oil, improve the safety of Antarctic krill oil products, and lay the foundation for the industrial production of high-quality Antarctic krill oil.
      Methods In this paper, frozen Antarctic krill powder was used as raw material. The response surface methodology was employed to optimize the extraction rate of Antarctic krill oil, while atomic fluorescence photometry was utilized to assess the effectiveness of arsenic removal from the oil. Building on single-factor tests, the extraction rate of Antarctic krill oil served as the response variable, with ethanol concentration, liquid-to-material ratio, and ultrasonic extraction time selected as independent variables. The extraction process of Antarctic krill oil was optimized, and the arsenic removal efficiency of the krill oil was evaluated.
      Results The results indicated that a quadratic polynomial regression equation has been established using the response surface analysis method: Xyield=19.02−0.30A+0.68B+0.92C−0.24AB−0.19AC+0.49BC−1.11A2−1.04B2−1.29C2. The model showed a significance level of P<0.0001, and the misfit term had P>0.05, indicating that the model was highly significant and relatively stable. Through model analysis, the optimal conditions were determined to be: ethanol concentration of 91.1563%, liquid-to-material ratio of 8.6892 mL/g, and ultrasonic extraction time of 47.4734 minutes. Considering practical application feasibility, these conditions were adjusted to an ethanol concentration of 91%, a liquid-to-material ratio of 8 mL/g, and an ultrasonic extraction time of 47 minutes. Under the revised conditions, the actual yield of Antarctic krill oil was found to be 18.36%±0.58%, which was in close agreement with the theoretical prediction of 18.62% from the model. The Cleanert Alumina A SPE column with activated alumina as the medium could remove 82.61%±4.63% of the total arsenic from Antarctic krill oil.
      Conclusion Based on the response surface methodology, the extraction process for Antarctic krill oil involved using 91% ethanol, a liquid-to-material ratio of 8 mL/g, and an ultrasonic extraction time of 47 minutes. Cleanert Alumina A adsorption columns using activated alumina as a medium can be used as an ideal material for arsenic removal of Antarctic krill oil in the future.

       

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