Abstract: This study aimed to investigate the low-salt tolerance of various larval offspring of Litopenaeus vannamei A,B,C,and D groups from inbreeding and/or hybridization so as to discover technologies of selection and breeding.The larvae used for study were in the growth period of P₁₁~P₁₂ with body length of 6.09~7.54 mm.Over the experimental period of 48 h,the water temperature was at 25.0~29.3℃.The survival rate at 48 h and factors such as body length were used to evaluate the low salt tolerance of larvae from different provenances.The results showed that at salinity 3,among the 17 experimental groups,6 reporting lower survival rates(17.69%~57.37%) uncovered significant differences in survival rate of being tolerant to low salt(P<0.05) with the other 11 reporting higher ones(68.72%~96.10%). The 2 groups with the best tolerance to low salt were the progenies and backcrosses of group A and group D,respectively.The two with the lowest tolerance to low salt were the self-bred progenies of group D and group C.It was suggested that there were differences in the low salt tolerance of larval offspring from different provenances and/or different mating modes.The results could provide reference for prawn breeding research.