Abstract: Vomitoxin is a natural mold product,a major pollutant in feed and its raw materials,and a mycotoxin that has appeared most frequently in aquatic feed in recent years.In this study,a certain amount of Bacillus amyloliquefaciens(10⁶,10⁷,10⁸ CFU/mL),biological detoxifying enzymes,and biologically active agent T were used for combination compatibility.Under the condition of 28℃,the detoxifying efficiency of different combinations of compatibility on vomiting toxin was tested by enzyme-linked immunosorbent assay(ELISA)after in vitro culture for 16 h.The results showed that Bacillus amyloliquefaciens,biological detoxification enzyme and biologically active agent T all had a certain detoxification efficiency against the vomiting toxin.Among them,the detoxification efficiency of Bacillus amyloliquefaciens and biological detoxification enzymes combination was better,and the detoxification efficiency was the highest in 10⁶ CFU/mL Bacillus amyloliquefaciens.