Determination of 3,6-anhydro galactose in agar and agarose

This paper studied the determination of resorcinol method on agar and agarose samples of 3,6-anhydro galactose and optimize the determination wavelength, color temperature, color time and colorimetric time. the results show that the optimum condition is determination of wavelength of 554nm,color temperature 80℃,reaction time 15min; under this condition, agar and agarose samples of 3,6-anhydro galactose were measured ,the relative deviation of 0.94% to 3.0%, and the spiked recovery of 105.4% to 111%. It shows that the method is simple, high accuracy and repeatability.