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JOURNAL OF FUJIAN FISHERIES ›› 2013, Vol. 35 ›› Issue (2): 118-123.

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Detection of malachite green residue in fish by ELISA


  • Received:2012-12-20 Revised:2013-01-22 Online:2013-04-25 Published:2013-04-27



  • 通讯作者: 万宇平

Abstract: We developed a kit for determine the malachite green(MG) and crystal violet residues in fish by enzyme-linked immune-sorbent assay(ELISA) and had a quality verification.The results show that the linear range was from 0.2 ng/mL to 5.4 ng/mL, the IC50 was 0.495 ng/mL, and the detection limits of MG in fish was 0.17 ng/g. The average recovery rate was higher than 72.4%, the intra-assay and inter-assay coefficient of variation(CV) were 12.4% and 12.9% respectively. This method is sensitive, accurate and rapid, suitable for the determination of malachite green, crystal violet, leucomalachite green and leucocrystal violet total residues in fish.

摘要: 开发了一种用于检测鱼肉中孔雀石绿(MG)和结晶紫的间接竞争酶联免疫(ELISA)检测试剂盒,并对试剂盒性能进行了验证。结果表明,MG浓度在0.2~5.4ng/mL范围内有较好线性关系,50%竞争抑制率为0.5 ng/mL,试剂盒检测鱼肉中MG的最低检测限为0.17 ng/g。鱼肉中分别添加0.2、0.4、0.8 ng/g的MG标准品,回收率均在72.4%以上,批内变异系数小于12.4%,批间变异系数小于12.9%。本方法灵敏度高,重复性好,检测时间短,可同时检测鱼肉中孔雀石绿、结晶紫、隐性孔雀石绿、隐性结晶紫等四种药物的残留总量。

关键词: 孔雀石绿, 结晶紫, 酶联免疫法, 鱼, malachite green, crystal violet, enzyme-linked immune-sorbent assay, fish