Abstract: Rinse is one of the important steps of minced fish processing. Lots of soluble protein would be lost in the process of rinsing. So, recovering protein from the minced fish rinse solution can improve the utilization rate of the surimi, reduce the cost of sewage treatment steps, and decrease the pollution of environment. In this paper, FeCl3 as a flocculant was used to retrieve the water soluble protein from the surimi rinse water. According to different single factor variables, the optimal conditions of the recovery of protein from the surimi rinse water by FeCl3 flocculation method were researched and optimized by response surface analysis. Results showed that the optimal conditions of FeCl3 recovery proteins were: pH 6, temperature of 65℃, and an addition amount of 1% FeCl3 solution of 1.4 mL in 10 mL minced fish rinse solution.