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›› 2019, Vol. 41 ›› Issue (6): 514-518.

• 论文与报告 • 上一篇    下一篇

电刺激获得观赏螯虾天空蓝魔精荚条件的优化

洪伟1,王国栋2,宋紫玉1,谯江华1   

  1. 1. 福建省厦门市集美区集美大学水产学院
    2. 集美大学水产学院
  • 收稿日期:2019-07-29 修回日期:2019-10-05 出版日期:2019-12-25 发布日期:2019-12-26
  • 通讯作者: 王国栋
  • 基金资助:
    福建省种业创新与产业化工程

Optimization of the conditions for obtaining spermatophore of yabby by electrical stimulation

  • Received:2019-07-29 Revised:2019-10-05 Online:2019-12-25 Published:2019-12-26

摘要: 电刺激天空蓝魔(Cherax destructor)成熟雄性的第五步足基部,能够较专一地引起输精管附近肌肉的收缩,以此获得精荚。本文在不损伤虾体的情况下,以获取更多正常精荚为目的,对电刺激的电压、频率和刺激时间等条件进行优化。试验结果显示:刺激电压为45V、频率为32Hz、持续刺激时间为4s,为最优刺激条件,这时能够达到75%的精荚排出率,且精荚及精荚内精细胞形态正常。精荚内精子数量在5.2×104~1.13×106之间。本次试验获取的精荚可作为人工授精的材料,为天空蓝魔的人工繁殖提供技术支撑。

关键词: 电刺激, 天空蓝魔螯虾, 精荚, electrical stimulation, Cherax destruct, spermatophore

Abstract: Electric stimulation of the male Cherax destructor's fifth foot base and front and rear can specifically cause contraction of the muscle near the vas deferens and can be used to obtain spermatic pods. In this paper, the voltage, frequency, duration and other conditions of electrical stimulation were optimized for the purpose of obtaining more normal sperms without damaging the shrimp body. The test results show that the stimulation voltage is 45V, the frequency is 32hz, and the stimulation time is 4s, which is the optimal stimulation condition. The spermatozoa in spermata were mature and normal, and the number of spermatozoa in a single spermatozoa ranged from 5.2×104 to 1.12×106. The pods obtained in this experiment can be used as artificial insemination materials to provide technical support for the artificial reproduction of blue devils in the sky.