关键词: 珍珠龙胆石斑鱼幼鱼, 溃疡病, 哈维氏弧菌, 鉴定, juvenile pearl gentian grouper (E.pinephelus fuscoguttatus♀×E. lanceolatus♂), Vibrio harveyi, skin ulcer disease, identification

Abstract: A bacterial strain, named HW2018, was isolated from juvenile pearl gentian grouper (Epinephelus fuscoguttatus♀×E. lanceolatus♂) with skin ulcer disease and identified as Vibrio harveyi by morphological observation, physiological and biochemical characteristics and phylogenetic analysis of 16S rDNA sequences. Artificial infection experiment proved that the strain HW2018 was the causative pathogen. Death rate in one week was 100% with the injection dose (3×104 cfu/g) through celiac injection or muscle injection. The drug sensitivity test revealed that V. harveyi was sensitivity to meropenem, ciprofloxacin and levofloxacin, but resistant to ampicillin.