Abstract：Clostridium butyricum is a gram-positive, obligate anaerobic and endospore-forming probiotic，which has a high adapting ability to higher temperature and lower pH. As a new kind of microbial feed additive, it has been studied and applied in livestock and poultry breeding，but its application in aquaculture is rarely reported . C. butyricum has the function of maintaining the ecological balance in digestive tract, repairing the intestine epithelium, improving the immunity of the organism and promoting the absorption of nutrients in aquaculture. In this paper, we reviewed advances in the biological function and the application in aquaculture of C. butyricum, and pointed out problems during the application of C. butyricum as feed additive. Some advices were also put forward for further research of C. butyricum.