欢迎访问《渔业研究》官方网站,今天是 分享到:

›› 2018, Vol. 40 ›› Issue (4): 258-267.

• 论文与报告 • 上一篇    下一篇

时间分辨免疫荧光层析快速检测水产品中高效氯氰菊酯方法的建立

姜琳琳,苏捷   

  1. 福建省水产研究所
  • 收稿日期:2018-06-15 修回日期:2018-07-06 出版日期:2018-08-24 发布日期:2018-08-21
  • 通讯作者: 姜琳琳
  • 基金资助:
    福建省公益类科研院所基本专项;福建省海洋高新产业发展专项

Time-resolved fluorescent immunochromatographic assay for cypermethrin in aquatic product

,   

  • Received:2018-06-15 Revised:2018-07-06 Online:2018-08-24 Published:2018-08-21

摘要: 高效氯氰菊酯在农业中应用广泛,其中水产品可以富集水系统中的高效氯氰菊酯,并通过食物链,影响人体的健康。本研究通过铕(Eu)颗粒标记氯氰菊酯抗体,研发针对高效氯氰菊酯检测的时间分辨免疫荧光检测卡。在对虾样品中添加高效氯氰菊酯标准品分析检测卡的准备度、精确度等指标时,研究发现氯氰菊酯检测卡的平均相对误差在4.24% ~8.66%之间,检测卡的批内变异系数在3.0%~8.6%之间,批间变异系数在4.8%~6.6%之间,最低检测限为0.35 ng/mL,检测范围为1~19.3 ng/mL,四参数拟合曲线决定系数R2=0.999 0;检测卡检测结果与气相色谱检测结果的相对误差在8 %~20 %之间,线性相关系数R=0.989 9。结果表明本技术方法可以应用于水产品中高效氯氰菊酯的快速定量检测。

关键词: 高效氯氰菊酯, 时间分辨免疫荧光, 快速定量检测。, cypermethrin, time-resolved fluorescent immunochromatographic assay, rapid quantitative detection.

Abstract: Cypermethrin is widely used in agriculture. Aquatic products can enrich cypermethrin in the water system, and then affect the health of human through the food chain. In this study, the time-resolved fluorescent immunochromatographic assay for cypermethrin was developed through using Eu particles to mark cypermethrin antibody. The results showed that the average relative error of cypermethrin detection card was between 4.24% and 8.66%; the detection range of cypermethrin card was 1-19.3 ng/mL in the shrimp samples; the minimum detection limit was 0.35 ng/mL; the coefficient of determination R2 was 0.9990; the intra batch variation coefficient of the detection card was between 3.0%~8.6% and the inter batch variation coefficient was between 4.8%~6.6%; the relative error between the detection card detecting result and the gas chromatography results was between 8%~20%, and the linear correlation coefficient R was 0.989 9. In conclusion, the time-resolved fluorescent immunochromatographic assay for cypermethrin can be applied to the rapid quantitative detection of cypermethrin in aquatic products.

中图分类号: