关键词: 波纹巴非蛤, 组织切片, 性腺发育, 人工育苗, Paratapes undulatus, microsection observation, gonadal development, artificial breeding

Abstract: Paratapes undulatus is an important commericial shellfish in the coastal areass of Fujian Province. However, the reduction of natural resources has become a bottleneck restricting the development of the aquaculture industry. In order to guide the artificial breeding of this species, in this study, gonad annual development was determined by microsection observation and artificial spawning breeding experiments were also performed. Microsection observations indicated that the gonad development of P. undulatus could be divided into five periods, e.g., resting stage (I), proliferative stage (II), growing stage (III), mature stage (IV) and emission stage (V). The maturation rate (R) of gonad reached its peak in July (R=0.93). Between April and November, we conducted one or two artificial spawning tests every month, and the data showed that the spawning effects in June and July were the best, followed by September and October. Combining gonad annual development changes, artificial spawning situations and sea water temperature, this study obtained important basic data that would benefit local artificial breeding and natural resource protection for P. undulatus.