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渔业研究 ›› 2018, Vol. 40 ›› Issue (1): 16-23.

• 论文与报告 • 上一篇    下一篇

海南省6个养殖罗非鱼群体遗传差异的微卫星分析

佟延南1,李高俊2,李芳远1,赵光军2,王德强1   

  1. 1. 海南省海洋与渔业科学院
    2.
  • 收稿日期:2017-10-30 修回日期:2017-12-18 出版日期:2018-02-26 发布日期:2018-03-05
  • 通讯作者: 王德强

Microsatellite analysis of the genetic differences among six species of farmed tilapia from Hainan Province

  • Received:2017-10-30 Revised:2017-12-18 Online:2018-02-26 Published:2018-03-05

摘要: 利用筛选的20对微卫星引物对海南省6个养殖罗非鱼群体进行遗传多样性研究,分析各群体内的遗传变异和各群体间的遗传关系,比较不同群体的等位基因数、有效等位基因数、观测杂合度、期望杂合度、Shannon’s 多态性指数、平均多态性信息含量等遗传参数,并利用遗传距离构建6个罗非鱼群体的系统聚类图。结果表明:1)6个罗非鱼群体等位基因数为68个,平均等位基因数为1.992 2~2.255 8,平均观测杂合度为0.5725~0.7450,平均期望杂合度为0.469 2~0.532 3, Shannon’s多样性指数为0.719 3~0.848 6,多态信息含量为0.25

关键词: 罗非鱼;微卫星;遗传差异, tilapia

Abstract: Genetic diversity of six species of tilapia in Hainan province was studied with twenty pairs of previously screened microsatellite primers to analyze the genetic variation and genetic relationships among these groups. The genetic parameters in different groups were also compared, which included allele number, efficient allele number, observation heterozygosity, expectation heterozygosity, Shannon's polymorphism index, average polymorphic information content, etc. A systematic cluster map of six tilapia groups was constructed using genetic distance. The results showed that the number of allele of the 6 groups was 68. The average allele number was 1.992 2~2.255 8. The average observed heterozygosity was 0.572 5~0.745 0. The average expected heterozygosity was 0.469 2~0.532 3. The Shannon's diversity index was 0.719 3~0.848 6. The polymorphic information content got 0.25