关键词: 棘胸蛙, 高温胁迫, 乳酸脱氢酶（LDH）, 谷胱甘肽过氧化物酶（GSH-Px）, 超氧化物歧化酶（SOD）, 过氧化氢酶（CAT）, 碱性磷酸酶（AKP）, 丙二醛（MDA）, Quasipaa spinosa, heat stress, lactate dehydrogenase(LDH), glutathione peroxidase(GSH-Px), superoxide dismutase(SOD), catalase(CAT), alkaline phosphatase(AKP), malondialdehyde (MDA)

Abstract: The activity of LDH, GSH-Px, SOD, CAT, AKP and the content of MDA of the frog tadpole (Quasipaa spinosa) were detected under raising water temperature from18℃ to 30℃ with rapid and gentle models. The results showed the activity of LDH extracted from the tail muscle tissue were significantly higher than that of extracted from visceral mass of the frog tadpole（P<0.01）; under heat stress, the activities of LDH extracted from the tail muscle tissue and visceral mass of the frog tadpole were significantly higher （P<0.01）. The activities of GSH-Px of the tail muscle tissue and visceral mass of the frog tadpole were significantly lower under heat stress (P<0.01, P<0.05), and the GSH-Px activities of the visceral mass of the tadpole in the rapid raising temperature model were higher than that of gentle raising temperature model significantly (P<0.01). Under heat stress, the activities of SOD extracted from the tail muscle tissue and visceral mass of the frog tadpole were significantly lower（P<0.05,P<0.01）, and the SOD activities of the tail muscle tissue and visceral mass of the tadpole in the rapid raising temperature model were higher than that of gentle raising temperature model significantly (P<0.05,P<0.01). Under heat stress, the activities of CAT extracted from the tail muscle tissue and visceral mass of the frog tadpole were significant drop（P<0.01）, and under gentle raising temperature model, the activities of CAT extracted from the tail muscle tissue of the tadpole were higher than that of rapid raising temperature model significantly (P<0.05,P<0.01). There were no obvious effects of heat stress on AKP activity of the tadpole tail muscle under the gentle and rapid models of raising water temperature. There were significant rise on AKP activity of the visceral mass of the tadpole under heat stress（P<0.05）. There were significant rise on MDA contents of the tail and the visceral mass of the tadpole under heat stress（P<0.05）, and the MDA contents of the visceral mass of the tadpole in the rapid raising temperature model were higher than that of gentle raising temperature model significantly（P<0.01）.