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›› 2016, Vol. 38 ›› Issue (1): 27-35.

• 论文与报告 • 上一篇    下一篇

云纹石斑鱼(♀)×鞍带石斑鱼(♂)杂交子代胚胎发育及仔稚幼鱼形态观察

吴水清1,郑乐云1,黄种持1,仇登高2,许朝辉2,郑君飘2,邱峰岩2,吴精灵2,刘银华2   

  1. 1. 福建省水产研究所
    2.
  • 收稿日期:2015-12-30 修回日期:2016-01-20 出版日期:2017-02-27 发布日期:2016-04-15
  • 通讯作者: 郑乐云

Embryonic and morphological development in larva, juvenile, and young stages of hybrid grouper (Epinephelus moara ♀×E. lanceolatus ♂)

  • Received:2015-12-30 Revised:2016-01-20 Online:2017-02-27 Published:2016-04-15
  • Contact: Le-Yun ZHENG

摘要: 对云纹石斑鱼(♀)与鞍带石斑鱼(♂)杂交子代胚胎及仔稚幼鱼的生长发育进行了观察与研究。在水温(22 ±1)℃、盐度33、pH7.8的海水中,杂交受精卵经过35 h 23 min完成整个胚胎发育。胚胎发育可分为卵裂期、囊胚期、原肠期、神经胚期和器官形成期,其中在卵裂期、囊胚期和仔鱼出膜前后,杂交子代胚胎的死亡率较高。杂交石斑鱼的孵化率为91.5%,低于对照组的93.1%;畸形率为10.5%高于对照组的5.3%。根据杂交子代卵黄囊消失与否、第二背鳍棘和腹鳍棘的生长与收缩、体色和鳞片的出现,可将杂交石斑鱼胎后发育分为:前期仔鱼(0?3日龄)、后期仔鱼(4?27日龄)、稚鱼期(28?45日龄)、幼鱼期(46日龄以后);通过与其亲本早期发育进行比较发现,云纹石斑鱼(♀)与鞍带石斑鱼(♂)杂交子代具有一定的杂交优势。

关键词: 云纹石斑鱼, 鞍带石斑鱼, 杂交子代, 胚胎发育, 形态观察, Epinephelus moara, Epinephelus lanceolatus, hybride, embryonic development, morphological observation

Abstract: Hybridization is the most widely used and effective method of artificial breeding in aquaculture. We documented embryonic development and the growth and morphological characteristics of larva, juveniles, and young fish in the hybrid (Epinephelus moara ♀× E. lanceolatus ♂) by artificial methods. The embryonic development of the hybrid was divided into cleavage stage, blastula stage, gastrula stage, neurula stage, and organogenesis stage. In sea water (22±1℃, salinity 33, pH7.8), 35 h 23 min was required for hybrid embryonic development. In cleavage stage, blastula stage, before and after the larvae, the hybrid had higher mortality. Statistics showed that hybrid hatching rate was 91.5% lower than control group (93.1%), deformity rate was 10.5% higher than control group (5.3%). Based on the features of the yolk sac, second dorsal fin spine, pelvic fin spine, scales and body color, the post-embryonic development of the hybrid was divided into larval (0~27 d), juvenile(28~45 d), and young fish stages(46 d). The results suggested that the hybrid grows more rapidly than E. moara.