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›› 2016, Vol. 38 ›› Issue (1): 21-26.

• 论文与报告 • 上一篇    下一篇

一例引起南日鲍死亡的病原分离与鉴定

严志洪   

  1. 莆田市秀屿区水产技术推广站
  • 收稿日期:2015-06-15 修回日期:2015-07-24 出版日期:2017-02-27 发布日期:2016-04-15
  • 通讯作者: 严志洪

Isolation and identification of a pathogen from Haliotis discus nanri

  • Received:2015-06-15 Revised:2015-07-24 Online:2017-02-27 Published:2016-04-15

摘要: 观察了一例南日鲍( Haliotis discus nanri)病鲍的症状,从病症明显的濒临死亡的南日鲍病鲍足部病灶区、消化腺中分离到一株优势菌,对该优势菌进行了浸浴感染和注射感染的回归感染试验、生理生化特征测定及药敏试验。结果表明:南日鲍发病初期,没有明显的症状,发病持续一段时间后足部表面有大小不一的溃烂孔;回归感染试验结果表明该优势菌为致病菌,经生化指标的测定,确定该致病菌为溶藻弧菌(Vibrio alginolyticus);该菌对氟甲喹和氟苯尼考等21种抗菌药物敏感。本研究结果为南日鲍病害防治提供一些基础数据。

关键词: 南日鲍, 溶藻弧菌, 药敏试验, Haliotis discus nanri, Vibrio alginolyticus, drug susceptibility tests

Abstract: The occurrence of disease is becoming more and more serious with the expansion of Haliotis discus nanri breeding scale, but so far no special reports focus on the research of these diseases. In order to provide some basic information for disease prevention and control of H. discus nanri, a dominant bacteria strain was isolated from the foot lesions and digestive gland of obviously dying individuals which were showing overt symptoms and proved to be the pathogen by recurrent infection experiment. The disease symptom of the pathogenic bacteria was observed carefully while biochemical characteristics and drug susceptibility test of the pathogenic bacteria were also detected separately. It turned out that the disease symptom was not significantly at the initial stage, and festering holes of different sizes could be just found on the foot of H. discus nanri after a period of time. The results of physiological and biochemical analyses showed the pathogen was Vibrio alginolyticus which was sensitive to 21 kinds of antibiotics such as flumequine and florfenicol. It highly recommend that drug sensitive experiment should be necessary to carry out every time with the purpose of screening out the appropriate effective drugs to prevent and control the V. alginolyticus in H. discus nanri production process.