关键词: 凡纳滨对虾, 育苗, 生物絮团, 细菌群落, 宏基因组测序, Litopennaus vannamei, larval rearing, biofloc, bacterial community structure , metagenome sequencing

Abstract: During larval rearing of Litopenaeus vannamei, the formation of biofloc in culture pond water could be facilitated after adding sucrose as the main carbohydrate sources. The analysis of biofloc taxonomic composition of metagenomic community showed that the species abundance and dominant bacteria in biofloc were fundamentally the same within experimental and control group in the level of phylum, class and genera, but the proportion of bacteria composition was different. The proportion of Alphaproteobacteria detected in experimental and control groups, which have the ability of removing contaminants, was 16.2% and 14.4%, respectively. The proportion of Pseudoalteromonas and Vibrio, which were the common pathogenic bacteria, was 1.3% and1.4% together with 0% and 0.1% in experimental and control groups. The paper provided the basic data for the application of biofloc technology in the process of shrimp nursery and breeding.