关键词: 西施舌, 铜, 蓄积, 超氧化物歧化酶, 过氧化氢酶, Coelomactra antiquata, copper, accumulation, SOD, CAT

Abstract: For the purpose of investigating the accumulation of Cu2+ and the effect on the activity of SOD and CAT in gill and digestive gland of Coelomactra antiquata，the acute toxicity experiment was adopted in this paper. C. antiquata was set in the experimental groups with the different concentration of Cu2+. The result showed that the maximum of Cu accumulation in gill was 79. 7 ug/g in the 4th day. The maximum of Cu accumulation in digestive gland was 139.2 ug/g in the 8th day. The maximum accumulation of Cu both appeared in the test group of 0. 16 mg/L. The increase of Cu accumulation in digestive gland accompanied with the higher doses of Cu2+. The activity of CAT in both tissues showed a tendency to increase at first and then decrease. The activity of CAT in digestive gland was higher than that in gill. The S O D activity in gill also trended to increase at first and then decrease， which showed significant difference with the control group (P<0.05) . The activity of SOD in digestive gland was decreased with exposure time in treatment groups. The activity of SOD of both gill and digestive gland in the group of 0.16 mg/L was depressed with extremely significant difference（P<0.01) in the 3rd days and the 4th days,respectively. CAT and SOD could be used as the important biomarker of Cu pollution for C. antiquata.