关键词: 羊栖菜, 海藻, 无机砷, 高效液相色谱-氢化物发生-原子荧光联用法, Sargassum fusiforme, seaweeds, inorganic arsenic, HPLC-HG-AFS

Abstract: This paper is about the research of the determination of inorganic arsenic in edible seaweeds with High performance liquid chromatography-hydride generation-atomic fluorescence spectrometry(HPLC-HG-AFS). The curved surface fitting method was applied to establish optimum conditions of extraction reagent, time and temperature based on the inorganic arsenic contents of Sargassum fusiforme. It was found that the optimum conditions is with 0.1%HCL at 52.5℃ for 76min. The detection limit of methodology was 0.0635mg/kg. The recovery rates of 1.0 mg/kg and 10.0mg/kg in samples were between 80.5% and 105.7% and the relative standard deviations were between 4.5% and 5.0%. The content of inorganic arsenic of sixty types of laver from different areas in Fujian province were examined. The results showed that inorganic arsenic concentration in laver was below，which accounted for the total arsenic concentration of 0～1.96%.