Abstract��27 strains of Aeromonas hydrophila were isolated from cultured fish in Fujian Province��Based on the 16S rDNA gene sequence and aerolysin gene sequence of A. hydrophila��the synthetic oligonucleotide primers were designed and used to perform duplex PCR amplification of the two conservative genes fragments��The resuts showed that 18 strains were pathogenic A. hydrophila��Its was 66.67% Of the strains isolated. Enterobacterial repetitive intergenic consensus sequence PCR(ERIC-PCR) was used to genotype 27 strains of A. hydrophila, By the similarity 54.00%��two special genotypes (��and ��)from the 27 isolated were identified. However,All the strains could be divided into eleven groups by the similarity 76.00%.There was a tendency that the strains from the same area could be clustered into one group. The results showed Its likely a correlation between the genotype and isolating regional among A. hydrophila strains. And ERIC-PCR was of a great significance for Molecular epidemiological investigation of A. hydrophila��