Abstract��Portuguese oyster larvae were exposed to solutions of KCl��CaCl2��MgCl2��NH4Cl at various concentrations. The result is showed below. At the beginning of the test, K+ and NH4+ induced larvae settlement behavior. After a week, except Mg2+ and NH4+, under the concentration of 10mM,20mM, K+ and 10mM-25mM, Ca2+ both induced larvae settlement and metamorphosis; NH4+ caused high mortality. This indicated that in commercial breeding of Portuguese oyster, KCl��CaCl2 may be the potential chemical cues.