关键词: 人工移植精荚技术, 斑节对虾, 育苗生产, 应用, artificial spermatophore transplantation technology, penaeus monodon:nursery production, application

Abstract: This paper described the application of artificial spermatophore transplantation in Penaeus monodon nursery production. The results showed that the artificial spermatophore transplantation could let female shrimp fertilize repeatedly and fully utilized the expensive parent shrimp, and thereby reduce the nursery costs and increase the production. The artificial spermatophore transplantation technology includes spermatophore obtainment and implantation. Among three methods for spermatophore obtainment including extraction, extrusion and cannulation, the method of extraction was more convenient and effective since the spermatophore could regenerate and become matured after extraction. While the methods of extrusion and cannulation had greater impact on parent shrimp and the success rate was not high. A spermatophore was implanted in every transplantation implantation procedure by single person operation. The best time for obtaining the highest success rate of artificial spermatophore transplantation for Penaeus monodon was within 12 to 24 h after shelling. After 4 or 5 days of intensive culture, the ovary of parent shrimps could fully grow and spawn again, and the offsprings were without obvious different from those of non-surgical parent shrimp breeding in larvae hatch and development, the natural mating can be obtained.