欢迎访问《渔业研究》官方网站,今天是 分享到:

福建水产 ›› 2012, Vol. 34 ›› Issue (4): 290-295.

• 研究论文 • 上一篇    下一篇

胶体金免疫层析法快速检测水产品中呋喃妥因代谢物残留

吴茂生1,何方洋2,赵正苗3,崔廷婷2,汪善良3,刘婷婷3   

  1. 1. 福建省海洋环境与渔业资源监测中心
    2. 北京勤邦生物技术有限公司
    3.
  • 收稿日期:2012-05-14 修回日期:2012-06-04 出版日期:2012-09-25 发布日期:2012-08-21
  • 通讯作者: 何方洋

Gold immunochromatographic assay for rapid determination of nitrofurantoin metabolite residues in aquatic products

  • Received:2012-05-14 Revised:2012-06-04 Online:2012-09-25 Published:2012-08-21

摘要: 采用胶体金免疫层析技术(GICA)测定水产中的呋喃妥因代谢物残留,并用液相色谱-串联质谱(LC-MS/MS)对试验结果进行确证。结果显示,胶体金免疫层析法的检测限为1.0μg/kg,假阳性率不大于5%,假阴性率为0;对实际样本的检测结果与LC-MS/MS法一致。研究表明,胶体金试纸条法操作简便、快速,成本低,为水产中呋喃妥因代谢物的残留检测提供了快速筛选方法,而LC-MS/MS法灵敏度高,结果准确,适用于阳性样本的确证和精确定量。

关键词: 呋喃妥因代谢物, 水产, 胶体金免疫层析法(GICA), 液相色谱-串联质谱法(LC-MS/MS), nitrofurantoin metabolite (AHD), aquatic products, gold immunochromatographic assay (GICA), liquid chromatography-tandem mass spectrometric (LC-MS/MS)

Abstract: Gold immunochromatographic assay (GICA) was used to determine the nitrofurantoin metabolite (AHD) residues in aquatic products, while using liquid chromatography-tandem mass spectrometry (LC-MS/MS) to validate the results. The detection limit of GICA was 1.0 μg/kg. The false positive rate was less 5%, and false negative rate was 0. The results of the two methods for aquatic products were in accordance. The results showed that GICA has the advantages of convenient operation, rapidity and low cost, which provides a rapid method for the screen of AHD in aquatic products, while LC-MS/MS is accurate and suitable for determination of positive samples.