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福建水产 ›› 2012, Vol. 34 ›› Issue (2): 99-104.

• 研究论文 • 上一篇    下一篇

日本囊对虾精巢发育过程中组织器官生化组分含量的变化

姚东明1,吴超2,黄晶晶2,林琼武3,李少菁2   

  1. 1. 厦门大学海洋与环境学院海洋技术与工程系
    2.
    3. 厦门大学海洋与地球学院
  • 收稿日期:2011-12-23 修回日期:2012-01-26 出版日期:2012-04-25 发布日期:2012-05-11
  • 通讯作者: 林琼武

Change of biochemical component content of tissue and organ during the development of spermary of Marsupenaeus japonic

  • Received:2011-12-23 Revised:2012-01-26 Online:2012-04-25 Published:2012-05-11

摘要: 本实验采用酶学和现场采样等方法研究日本囊对虾精巢发育过程中精巢与肝胰腺的形态学和几种生化组分含量的变化,旨在说明海区自然状态下日本囊对虾亲虾生殖过程营养物质在组织器官中的消长与转移的情况。结果表明,台湾海峡海区自然状态下日本囊对虾精巢发育过程中,精巢指数从I期0.010±0.001~IV期0.017±0.002呈逐渐上升(P<0.05);肝胰腺指数从I期0.021±0.002~IV期0.025±0.003,虽也呈增大之势,但变化不大(P>0.05)。葡萄糖、胆固醇和甘油三酯的含量(mg/dl)变化情况分别如下:在精巢中,为14.85±5.40~4.75±3.17、7.69±2.47~16.46±6.79和 15.85±4.56~36.78± 8.12;在肝胰腺中,为10.95±2.60~22.24±5.76、27.12±9.11~202.62±77.26和42.87±13.39~116.99±24.57;在血淋巴中,为6.38±1.44~11.25±3.08、53.77±32.10~155.32±62.55和13.67±7.06~79.56±38.19。本文还就对虾精巢发育过程中,性腺和肝胰腺的形态变化,以及肝胰腺、血淋巴、精巢中葡萄糖、胆固醇和甘油三酯的含量的消长与转移的情况进行了详细的讨论。

关键词: 日本囊对虾, 精巢, 肝胰腺, 生化组份含量, Marsupenaeus japonica, spermary, hepatopancreas, biochemical component content

Abstract: In this study, enzymatic, field sampling and other methods were used to study pancreatic morphology and several biochemical component content changes in spermary and liver of Marsupenaeus japonicus during the spermary development. It intended to illustrate in the natural state, the growth and decline and transfer condition of nutrition substances in the tissues and organs of Marsupenaeus japonica broodstock in reproductive process The results showed that in the natural state of the Taiwan Strait waters, Marsupenaeus japonicus, testis index from the phase I 0.010 ± 0.001~IV period of 0.017 ± 0.002 was gradually increased (P<0.05); liver and pancreas fell from Phase I of 0.021 ± 0.002~to stage IV 0.025 ± 0.003, while also showed increasing trend, but little change (P>0.05). Glucose, cholesterol and triglyceride levels (mg/dl) changed as follows: In the testis, is 14.85 ± 5.40 ~ 4.75 ± 3.17,7.69 ± 2.47~16.46 ± 6.79 and 15.85 ± 4.56~36.78 ± 8.12; in the liver pancreas, is 10.95 ± 2.60~22.24 ± 5.76,27.12 ± 9.11~202.62 ± 77.26 and 42.87 ± 13.39 ~ 116.99 ± 24.57; in the hemolymph, is 6.38 ± 1.44~11.25 ± 3.08,53.77 ± 32.10~155.32 ± 62.55 and 13.67 ± 7.06 ~79.56 ± 38.19. This paper also showed that during the development of Marsupenaeus japonicus spermary, how gonad、pancreas and liver changeed in morphological area. The situations of rise and fall with the transfer in liver, pancreas, blood, lymph, testis glucose, cholesterol and triglyceride were also discussed in detail.