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福建水产 ›› 2011, Vol. 33 ›› Issue (5): 24-30.

• 研究论文 • 上一篇    下一篇

海地瓜中具吸湿保湿特性的胶原蛋白肽提取工艺研究

朱洪珍,刘淑集,吴成业,苏永昌,王茵   

  1. 福建省水产研究所
  • 收稿日期:2011-07-27 修回日期:2011-08-23 出版日期:2011-12-26 发布日期:2011-12-31
  • 通讯作者: 吴成业

Study on Extracting Collagen Peptides with characters of moisture retention and absorption capacity from Acaudina molpadioides

  • Received:2011-07-27 Revised:2011-08-23 Online:2011-12-26 Published:2011-12-31
  • Contact: wu chengye

摘要: 本文研究了海地瓜胶原蛋白多肽的提取工艺。利用氯化钠溶液去除海地瓜中的非胶原蛋白,通过单因素实验得出去除非胶原蛋白氯化钠的最佳浓度和体积:浓度为9%,体积为10倍海地瓜质量的氯化钠溶液,浸泡24 h,可除去的非胶原蛋白占总蛋白的11.5%。通过单酶实验,选择AS.1398中性蛋白酶为实验用酶。以吸湿性和保湿性为指标,在单因素的基础上,采用正交实验对制备海地瓜胶原蛋白多肽的条件进行了优化,获得最佳工艺条件为:料液比为1:2、温度40℃、加酶量2400U/g、pH 7.5、时间3 h,胶原蛋白多肽的吸湿率和保湿率分别为6.11%和81.23%。

关键词: 海地瓜, 胶原蛋白多肽, 吸湿, 保湿, Acaudina molpadioides, collagen protein peptide, moisture-absorption, moisture-retention

Abstract: The extraction technology of collagen peptides from acaudina molpadioides was studied in this article. The non-collagen protein of acaudina molpadioides was separated by sodium chloride solution, using single factor experiment to validate the optimal conditions of sodium chloride solution including the concentration of 9%, 10 times volume of the quality of acaudina molpadioides, marinated for 24h. Under these terms, the proportion of separated non-collagen protein in the total protein accounted for 11.5%. Based on single enzyme factor experiment, AS.1398 neutral protease was selected to hydrolyze collagen protein of acaudina molpadioides. Considering the moisture-absorption and moisture-retention, orthogonal experiment was conducted to optimize the preparation of the collagen peptides from acaudina molpadioides, with the results of the solid/liquid ratio 1:2, temperature 40℃, amount of added enzyme 2400 U/g, pH 7.5, and reacting time 3h, the moisture absorbency and moisture-retention ability achieved to 6.11% and 81.23%, respectively.

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